Yamashita, Tamura, Oiwa, Kobata, Kuriyama, Mushino, Murata, Hosoi, Nishikawa, Hanaoka, and Sonoki: Successful Intrathecal Chemotherapy Combined with Radiotherapy Followed by Pomalidomide and Low-Dose Dexamethasone Maintenance Therapy for a Primary Plasma Cell Leukemia Patient

Successful Intrathecal Chemotherapy Combined with Radiotherapy Followed by Pomalidomide and Low-Dose Dexamethasone Maintenance Therapy for a Primary Plasma Cell Leukemia Patient

Abstract

Primary plasma cell leukemia (PPCL) is a rare aggressive variant of plasma cell disorder and frequently presents with extramedullary disease. Central nervous system (CNS) involvement with PPCL has an extremely poor prognosis. We describe a 46-year-old man with PPCL treated with a combination of lenalidomide, bortezomib, and dexamethasone as induction therapy following upfront allogeneic stem cell transplantation (allo-SCT). Despite achieving a very good partial response, the patient suffered from an isolated CNS relapse 12 months after allo-SCT. He was immediately started on concurrent intrathecal chemotherapy (IT) and cranial irradiation (RT). Subsequently, pomalidomide and low-dose dexamethasone (Pd) were given as maintenance therapy. He has been without CNS recurrence for more than 18 months. Our case suggests that concurrent IT and RT followed by Pd maintenance therapy may be an effective option to control CNS relapse of PPCL after allo-SCT.




Competing interest statement

Conflict of interest: the authors declare no potential conflict of interest.

Introduction

Primary plasma cell leukemia (PPCL) is a very aggressive form of plasma cell disorder.1 PPCL is very rare, reported in only 4-6% of patients with multiple myeloma (MM).2,3 PPCL is defined as the presence of more than 20% plasma cells in the peripheral blood and an absolute plasma cell count greater than 2 × 109/L, without previous evidence of MM.1 Consistent with the high tumor burden, extramedullary involvement, such as hepatosplenomegaly, lymphadenopathy, soft-tissue plasmacytomas, or leptomeningeal infiltration, is more frequent in PPCL than in MM. 1 Despite the development of new agents, such as proteasome inhibitors or immunomodulatory drugs (IMiDs), for plasma cell disorders, the median overall survival of PPCL is reported to be 12 months.4 Although PPCL is difficult to treat, high-dose melphalan followed by autologous stem cell transplantation (auto-SCT) and/or allogeneic stem cell transplantation (allo-SCT) seems to improve outcomes in younger selected patients.5-7 However, patients with PPCL who had central nervous system (CNS) relapse after auto- and/or allo-SCT were refractory to various treatments, and had an extremely poor prognosis.7-9 We herein present a PPCL patient with CNS relapse after allo-SCT who underwent concurrent intrathecal therapy (IT) and radiotherapy (RT) followed by maintenance therapy with pomalidomide and low-dose dexamethasone (Pd).

Case Report

A 46-year-old man without a previous significant medical history was referred to our hospital with complaints of anorexia and generalized fatigue. Physical examination revealed slightly pale conjunctivae and bilateral leg edema. Laboratory findings revealed significant abnormalities, including a white blood cell (WBC) count of 19.7×109/L with 45% plasma cells, a hemoglobin level of 11.6 g/dL, a platelet count of 7.0×109/L, a creatinine level of 7.63 mg/dL, and a LDH level of 301 IU/L (Table 1). On a peripheral blood smear, the plasma cells were medium-sized, with round nuclei, basophilic cytoplasm, and occasional cytoplasmic vacuoles (Figure 1A). The serum IgG level was 75.88 g/L, and the serum free light chain (FLC) k/λ ratio was 161.11 (Table 1). In this case, a M-component of the IgG-k type was identified by serum protein electrophoresis. Moreover, flow cytometry on the peripheral blood showed that these plasma cells were positive for CD38, CD49e, CD54, CD138 and cytoplasmic kappa light chain. Thus, he was diagnosed with IgG-k PPCL. Later, cytogenetic analysis revealed 46,XY,der(14)? t(11;14)(q13;q32) in 9 metaphase cells (Figure 1B) and 46,XY in 11 metaphase cells, and a fluorescent in situ hybridization analysis revealed CCND1/IGH gene fusion rearrangement.

The patient was admitted to an intensive care unit (ICU) for continuous hemodiafiltration (CHDF). On the same day, we started induction therapy with lenalidomide (15 mg/day orally on days 1-14), bortezomib (1.3 mg/m2 subcutaneously on days 1, 4, 8, and 11), and low-dose dexamethasone (20 mg/day orally on days 1, 2, 4, 5, 8, 9, 11, and 12), also known as RVD induction therapy, each for a 21-day cycle.10 Eight days after starting the induction therapy, the circulating plasma cells in the peripheral blood disappeared, and he was transferred from the ICU after withdrawal of CHDF. After a total of 3 cycles of chemotherapy, the laboratory abnormalities markedly subsided, consistent with a very good partial response (VGPR), because serum immunofixation was positive. The patient was discharged from our hospital. He and his family consented to undergo allo-SCT.

At our outpatient department, because of grade 2 peripheral neuropathy, he continued induction therapy with lenalidomide (25 mg/day orally on days 1-14), bortezomib (1.3 mg/m2 subcutaneously on days 1 and 8), and dexamethasone (20 mg/day orally on days 1 and 8) in each 21-day cycle. After a total of 6 courses, allo-SCT was performed with the bone marrow from an unrelated donor (HLA-A one allele mismatch). The conditioning regimen consisted of fludarabine 120 mg/m2 (30mg/m2 on day -5, -4, -3, and -2), melphalan 180 mg/m2 (90mg/m2 on day-4 and -3), and rabbit anti-thymocyte globulin 2.5 mg/m2 (1.25mg/m2 on day -2 and -1). For the prevention of graft-versus-host-disease (GvHD), tacrolimus was started from day -1, and methotrexate was given on days 1, 3, and 6. We observed neutrophil engraftment on day 13. Complete donor chimerism was detected in the bone marrow on day 29. During this admission, the patient showed no symptoms of acute GvHD. He was discharged without complications 2 months after allo-SCT. However, serum immunofixation was positive despite normal IgG and FLC levels; therefore, he was still considered to have a VGPR.

Two months after discharge, the patient developed cervical, mediastinal, and axillary lymphadenopathy. The serum Epstein–Barr virus (EBV) DNA load was 6.4 × 104 copies/106 WBCs. He was therefore diagnosed with EBV-associated lymphoproliferative disease and was immediately treated with 2 cycles of rituximab monotherapy, resulting in a complete response. Tacrolimus was discontinued on day 180, and there was no evidence of chronic GvHD. However, 6 months after allo-SCT, he had marked cytomegalovirus (CMV) antigenemia. He also developed bilateral CMV retinitis, which was successfully treated with intravenous foscarnet.

Our patient suddenly developed headache and vomiting 350 days after allo-SCT. We performed a lumbar puncture to suspect CNS relapse quickly. The protein level in the cerebrospinal fluid was 53 mg/dL, and the glucose level was 55 mg/dL; 17 monocytes per microliter were detected. Cytological examination revealed that some of these cells resembled plasma cells (Figure 2A). Polymerase chain reaction for EBV, CMV, and human herpesvirus-6 was negative. Magnetic resonance imaging (MRI) with gadolinium (Gd) enhancement revealed a small, enhanced nodule in the lateral medulla oblongata (Figure 2B, arrowhead). Enhanced computed tomography (CT) showed no extramedullary tumors. Serum and urinary protein immunofixation electrophoresis were positive despite normal IgG and FLC levels. Moreover, we still observed complete donor chimerism, without leukemic plasma cells, in his bone marrow. These findings were consistent with isolated CNS involvement by PPCL. The patient underwent 4 courses of weekly IT, consisting of methotrexate 15 mg, cytarabine 20 mg, and prednisolone 40 mg in combination with local cranial RT (5 fractions of 2 Gy for a total dose of 10 Gy). After this combined treatment, his neurological symptoms resolved and the plasma cells in the cerebrospinal fluid disappeared. To prevent CNS recurrence, we administered pomalidomide 4 mg/day on days 1–21, with 20 mg of dexamethasone weekly for each 28-day cycle.11 After the two cycles, our patient developed grade 4 neutropenia. Subsequently, a reduced dose of 2 mg was administered for 2 cycles, but grade 4 neutropenia occurred again. He resumed pomalidomide at 1 mg and continued the low dose without severe hematological abnormalities. Twelve months after the CNS relapse, both enhanced CT and brain MRI with Gd enhancement revealed no extramedullary tumors. In our outpatient follow-up, he maintained VGPR without major complications for more than 18 months after the diagnosis of CNS relapse.

Discussion

The prognosis of plasma cell disorder has improved following the introduction of new agents and the development of SCT, but a cure remains elusive. The long-term survival rate of PPCL is low due to the aggressive nature of the disease that comprises high tumor burden and extramedullary involvement. In the US, median overall survival of PPCL was 12 months in 2006-2009.4 Upfront allo-SCT is still one of the treatment options for young PPCL patients without major complications. In our study, we performed upfront allo-SCT with a myeloablative-conditioning regimen for our young patients without major complications. The Center for International Blood and Marrow Transplant Research (CIBMTR) conducted a retrospective SCT study in PPCL, and demonstrated high survival rates in an autologous group compared with those in an allogeneic group.6 In the CIBMTR study, non-relapse mortality in the allogeneic group was much higher at 3 years (42% vs. 5%), despite this group possessing a lower relapse rate (39% vs. 61%). Our patient survived for more than 2 years after allo-SCT, although he unfortunately suffered from CNS relapse on day 350. Consolidation and maintenance therapy after auto- and/or allo-SCT in plasma cell disorder is still controversial. However, some clinical trials have recently shown that lenalidomide maintenance after auto-SCT for MM significantly prolonged progression-free survival (PFS) and overall survival.12-14 Moreover, a prospective clinical trial by the Intergroupe Francophone du Myélome group showed that a bortezomibbased regimen followed by auto-SCT and subsequent maintenance therapy with lenalidomide, bortezomib, and dexamethasone for PPCL significantly improved PFS compared with those who received a reduced intensity conditioning allograft.7 In the future, auto-SCT and maintenance therapy combined with novel agents may be a treatment option for young PPCL patients.

The prognosis of plasma cell disorders with CNS involvement is extremely poor. In a retrospective study on CNS myeloma conducted in 12 Greek institutions, there was no significant difference in median survival of CNS myeloma (called post CNS-MM) between novel drug-treated patients and those given other treatments (4 months vs. 2 months).15 By multivariable analysis, extramedullary lesions, prior to treatment with novel drugs, and high LDH levels at myeloma diagnosis were statistically independent predictors of post CNS-MM survival. These findings corroborate our observations that our patient with CNS relapse of PPCL had an extremely short-term outcome. The frequency of CNS involvement is high among PPCL patients. It has been reported that 19% of post CNS-MM patients met the criteria for PPCL.15 Furthermore, some of these PPCL patients developed isolated CNS relapse during systemic remission after auto-SCT.8,9,15 In a prospective study by Royer et al., 2 PPCL patients experienced neuromeningeal relapse after autologous- and allo-SCT, respectively, and both patients soon died despite intrathecal chemotherapy.7 In the present case, although VGPR was maintained by RVD induction therapy followed by allo-SCT, isolated CNS relapse occurred within 1 year.

Several drugs used in multiple myeloma, including bortezomib and lenalidomide, do not efficiently cross the blood–brain barrier (BBB).15 Meanwhile, IMiDs such as thalidomide and pomalidomide effectively penetrate the BBB.15-17 Of note, BBB penetration of lenalidomide was 11% in a rhesus monkey model, whereas that of thalidomide was 42%.16 In a murine model, pomalidomide displayed 39% BBB penetration and high anti-tumor activity in the CNS.17 To the best of our knowledge, the anti-tumor effect of thalidomide is inferior to that of pomalidomide.18 Additionally, pomalidomide is more effective in ameliorating extramedullary involvement.19,20 In our case, concurrent IT and RT followed by Pd was initiated after the diagnosis of isolated CNS involvement after allo-SCT. In addition, maintenance therapy by Pd might prolong survival to prevent systemic recurrence, including CNS involvement, for more than 18 months. The role of pomalidomide for PPCL patients with CNS involvement should be further explored in clinical trials.

Conclusions

Our PPCL patient received RVD induction therapy followed by up-front allo-SCT. His relapse involved the CNS, predicting an extremely poor outcome. However, concurrent IT and RT followed by pomalidomide-based maintenance therapy well controlled his CNS recurrence and prolonged his survival. To find an optimal treatment for this rare but aggressive PPCL, prospective clinical trials for this disorder should take into account the importance of myeloma drug penetration across the BBB in order to enhance the treatment of CNS recurrence.

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Figure 1.

Giemsa stained peripheral blood showing many circulating plasma cells (A). The G-banding chromosomes revealed 46,XY,der(14)?t(11;14)(q13;q32) in 9 metaphase cells (B).

hr-2017-1-6986-g001.jpg
Figure 2.

Several plasma cells were observed in the cerebrospinal fluid of our patients (A). T1-weighted gadolinium contrast-enhanced magnetic resonance imaging showed an intrathecal tumor with a high signal intensity around the medulla oblongata (B, arrowheads).

hr-2017-1-6986-g002.jpg
Table 1.

Laboratory data of the patient at the first visit to our hospital.

Complete blood count
White blood cells 19.7×109/L Plasma cell 45.0%
Neutrophil 31.0% Red blood cells 3.56×1012/L
Myelocyte 2.0% Hemoglobin 11.6 g/dL
Lymphocyte 13.0% Hematocrit 32.3%
Monocyte 5.0% Reticulocytes 56×109/L
Eosinophil 4.0% MCV 90.7 fL
Chemistry
Creatinine 7.63 mg/dL Creatine kinase 377 IU/L
Blood urea nitrogen 81.2 mg/dL Total protein 11.5 g/dL
Sodium 126 mEq/L Albumin 3.6 g/dL
Potassium 5.2 mEq/L C-reactive protein 0.03 mg/dL
Chlorine 93 mEq/L Glucose (fasting) 88 mg/dL
Calcium 14.8 mg/dL 2-microglobulin 28.7 mg/dL
Phosphorus 5.2 mg/dL IgG 75.88 g/L
Aspartate transaminase 55 IU/L IgA 0.21 g/L
Alanine transaminase 46 IU/L IgM <0.05 g/L
Alkaline phosphatase 132 IU/L Free light κ chain 1450 mg/dL
γ-glutamyl transpeptidase 45 IU/L Free light λ chain 9.0 mg/dL
Total bilirubin 0.6 mg/dL κ/λ ratio 161.11
Lactate dehydrogenase 301 IU/L

[i] Ig: Immunoglobulin; MCV: mean corpuscular volume

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Copyright (c) 2017 Yusuke Yamashita, Shinobu Tamura, Takehiro Oiwa, Hiroshi Kobata, Kodai Kuriyama, Toshiki Mushino, Shogo Murata, Hiroki Hosoi, Akinori Nishikawa, Nobuyoshi Hanaoka, Takashi Sonoki

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