Cover Image

Acute monocytic leukemia diagnosed by flow cytometry includes acute myeloid leukemias with weakly or faintly positive non-specific esterase staining

Yuriko Zushi, Miho Sasaki, Ayano Mori, Toshiharu Saitoh, Takae Goka, Yumi Aoyama, Yuta Goto, Hiroko Tsunemine, Taiichi Kodaka, Takayuki Takahashi
  • Yuriko Zushi
    Departments of Cell Therapy, Shinko Hospital, Kobe, Japan
  • Miho Sasaki
    Departments of Cell Therapy, Shinko Hospital, Kobe, Japan
  • Ayano Mori
    Departments of Cell Therapy, Shinko Hospital, Kobe, Japan
  • Toshiharu Saitoh
    Departments of Cell Therapy, Shinko Hospital, Kobe, Japan
  • Takae Goka
    Departments of Laboratory Medicine, Shinko Hospital, Kobe, Japan
  • Yumi Aoyama
    Departments of Hematology, Shinko Hospital, Kobe, Japan
  • Yuta Goto
    Departments of Hematology, Shinko Hospital, Kobe, Japan
  • Hiroko Tsunemine
    Departments of Hematology, Shinko Hospital, Kobe, Japan
  • Taiichi Kodaka
    Departments of Hematology, Shinko Hospital, Kobe, Japan

Abstract

A diagnosis of acute monocytic leukemia(AML-M5) based on α-naphthyl butyrateesterase (α-NB) staining has some problems,because AML-M5 leukemic cells often showweak or faint positivity on α-NB staining. Inthese situations, some cases of AML-M5tend to be misdiagnosed as AML-M0. Therefore, we evaluated the significance ofweak or faint α-NB staining in AML-M5diagnosed by flow cytometry (FCM). Nineteen AML cases in which leukemic cellswere negative for naphthol AS-D chloroac-etate esterase staining were studied. ForFCM, we defined leukemic cells as having amonocytic nature when more than 10% ofthe leukemic cells were positive for at leastone of the following antigens: CD4, CD11c,CD14, and CD64. The monocytic naturedetermined by FCM was consistent with pos-itive or weak positivity on α-NB staining. Five of 6 cases in which leukemic cellsexhibited faint positivity for α-NB stainingcould be diagnosed as AML-M5 by FCM,while negative α-NB staining was consistentwith a diagnosis of AML-M0. These resultssuggest that AML-M5 should be taken intoconsideration even when leukemic cells arefaintly positive for α-NB staining.

Keywords

AML-M5, AML-M0, flow cytom-etry, α-naphthyl butyrate esterase staining, surface monocytic antigen

Full Text:

PDF
Submitted: 2017-10-05 09:20:40
Published: 2018-03-29 08:24:39
Search for citations in Google Scholar
Related articles: Google Scholar
Abstract views:
117

Views:
PDF
19

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM


Copyright (c) 2018 Yuriko Zushi, Miho Sasaki, Ayano Mori, Toshiharu Saitoh, Takae Goka, Yumi Aoyama, Yuta Goto, Hiroko Tsunemine, Taiichi Kodaka, Takayuki Takahashi

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
 
© PAGEPress 2008-2018     -     PAGEPress is a registered trademark property of PAGEPress srl, Italy.     -     VAT: IT02125780185