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Evaluation of an enzyme-linked immunosorbent assay using purified, deglycosylated histoplasmin for different clinical manifestations of histoplasmosis

Allan Jefferson Guimaraes, Claudia Vera Pizzini, Marcos de Abreu Almeida, José Mauro Peralta, Joshua Daniel Nosanchuk, Rosely Maria Zancope-Oliveira
  • Allan Jefferson Guimaraes
    Albert Einstein College of Medicine, United States
  • Claudia Vera Pizzini
    Fundação Oswaldo Cruz, Brazil
  • Marcos de Abreu Almeida
    Fundação Oswaldo Cruz, Brazil
  • José Mauro Peralta
    Universidade Federal do Rio de Janeiro, Brazil
  • Joshua Daniel Nosanchuk
    Albert Einstein College of Medicine, United States


Diagnosis of invasive fungal diseases remains problematic, especially in undeveloped countries. We have developed an enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to Histoplasma capsulatum using metaperiodate treated purified histoplasmin (ptHMIN). Our ELISA was validated comparing sera from patients with histoplasmosis, related mycoses, and healthy individuals. The overall test specificity was 96%, with sensitivities of 100% (8/8) in acute disease, 90% (9/10) in chronic disease, 89% (8/9) in disseminated infection in individuals without HIV infection, 86% (12/14) in disseminated disease in the setting of HIV infection and 100% (3/3) in mediastinal histoplasmosis. These parameters are superior to the use of untreated histoplasmin in diagnostic ELISAs. The high specificities, sensitivities, and simplicity of our ELISA support further development of a deglycosylated HMIN ELISA for clinical use and for monitoring the humoral immune response during therapy in patients with chronic and disseminated histoplasmosis.


, Antibody detection, histoplasmosis, serological diagnosis

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Submitted: 2009-11-30 23:29:04
Published: 2010-03-17 11:00:14
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