A rapid method for isolation of low-molecular-weight RNA from Arabidopsis using low salt concentration buffer

Han Cheng; Jing Gao; Zewei An; Huasun Huang

doi:10.4081/pb.2010.e14

A rapid method for isolation of low-molecular-weight RNA from Arabidopsis using low salt concentration buffer

https://doi.org/10.4081/pb.2010.e14

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Vol. 1 No. 2 (2010)

Submitted: 23 January 2010
Accepted: 27 July 2010

Published: 11 August 2010

Low molecular RNA, Arabidopsis

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Authors

We have developed a rapid extraction method using low salt concentration buffer for the isolation of low-molecular-weight RNA from Arabidopsis tissues. The method was quick and efficient, and the small scale extraction process took no more than 1 hour, while yield and RNA quality were comparable with those of previously reported. The LMW RNA isolated using this method was high quality, abundant in small RNA and free of high molecular weight RNA. This method can be used to extract low-molecular-weight RNA for the purpose of small RNA cloning and detection, and library construction.

Supporting Agencies

Rubber Researchi Insititute, Chinese Academy of Tropical Agricultural Science

Cheng, H., Gao, J., An, Z., & Huang, H. (2010). A rapid method for isolation of low-molecular-weight RNA from Arabidopsis using low salt concentration buffer. International Journal of Plant Biology, 1(2), e14. https://doi.org/10.4081/pb.2010.e14