Optimization of total RNA isolation method from the aromatic medicinal plant Artemisia annua L.


https://doi.org/10.4081/pb.2012.e7
Vol. 3 No. 1 (2012)
Submitted: 30 September 2011
Accepted: 23 July 2012
Published: 10 December 2012
Artemisia annua L., tissue cultured leaves, total RNA extraction, PCR
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Authors

  • Suganthi Appalasamy Plant Tissue and Cell Culture Laboratory, School of Biological Sciences, University of Sains Malaysia, Penang, Malaysia.
  • Ning Shu Ping Plant Tissue and Cell Culture Laboratory, School of Biological Sciences, University of Sains Malaysia, Penang, Malaysia.
  • Arvind Bhatt Plant Tissue and Cell Culture Laboratory, School of Biological Sciences, University of Sains Malaysia, Penang, Malaysia.
  • Ahmad Sofiman Othman Plant Tissue and Cell Culture Laboratory, School of Biological Sciences, University of Sains Malaysia, Penang, Malaysia.
  • Nad-Ali Babaeian Jelodar Plant Tissue and Cell Culture Laboratory, School of Biological Sciences, University of Sains Malaysia, Penang, Malaysia.
  • Chan Lai Keng
    merrilynchan@gmail.com
    Plant Tissue and Cell Culture Laboratory, School of Biological Sciences, University of Sains Malaysia, Penang, Malaysia.
This paper is the first report on the development of a protocol that allows rapid and simplified extraction of total RNA from Artemisia annua L., an aromatic medicinal plant. This innovative protocol ensures a consistently high quantity and good quality of total RNA without any contamination of polyphenols, polysaccharides and proteins. The total RNA obtained is also free of fungal RNA even when extracted from fungal infested plants. The extraction buffer used in the proposed modified protocol was made up of non-hazardous chemicals. High concentrations of polyphenols of A. annua L. could be successfully eliminated and the prepared total RNA could be used for downstream reactions.

Supporting Agencies


Appalasamy, S., Shu Ping, N., Bhatt, A., Othman, A. S., Jelodar, N.-A. B., & Lai Keng, C. (2012). Optimization of total RNA isolation method from the aromatic medicinal plant Artemisia annua L. International Journal of Plant Biology, 3(1), e7. https://doi.org/10.4081/pb.2012.e7

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