In vitro and in vivo lineage conversion of bone marrow stem cells into germ cells in experimental Azoospermia in rat


https://doi.org/10.4081/scs.2011.e15
Vol. 1 No. 1 (2011)
Submitted: 3 September 2011
Accepted: 11 October 2011
Published: 19 October 2011
azoospermia, germs cells, MSCs, spermatids, spermatocytes.
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PDF: 512
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Authors

  • Mohammed T. Abdel Aziz Unit of Biochemistry and Molecular Biology Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt.
  • Taymour Mostafa Andrology & Sexology, Faculty of Medicine, Cairo University, Cairo, Egypt.
  • Hazem Atta Unit of Biochemistry and Molecular Biology Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt.
  • Soheir Asaad Histology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
  • Hanan H. Fouad
    hananfouadbostamy24@hotmail.com
    Unit of Biochemistry and Molecular Biology Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt.
  • Gamal Mohsen Unit of Biochemistry and Molecular Biology Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt.
  • Laila Rashed Unit of Biochemistry and Molecular Biology Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt.
  • Dina Sabry Unit of Biochemistry and Molecular Biology Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt.
  • Mohammed Abbas Unit of Biochemistry and Molecular Biology Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt.
The present study is conducted to assess in vitro transdifferentiation of bone marrow derived mesenchymal stem cells (MSCs) into germ cells and in vivo transdifferentiation into spermatids and spermatocytes in the seminiferous tubules of azoospermic rats. Forty eight male rats were included in the study and were divided into: group 1; control rats, group 2; experimental azoospermic rats, group 3; azoospermic rats received undifferentiated MSCs. Group 4; azoospermic rats received transdifferentiated MSCs into germ cells. Quantitative real time PCR was conducted to assess gene expression of a primordial germ cell marker (VASA), stem cell specific markers (Oct4, SSEA-1 and SSEA-3), specific molecular markers of germ cells (c-Kit, Daz1); premeiotic marker (Daz1) and post-meiotic markers (c- Kit, Stra 8). Histopathological examination of rat testicular tissue was also conducted. Results revealed that 12 weeks after transplantation, fluorescent labeled MSCs were detected in the seminiferous tubules of the testes of group 3 and group 4 rats. In group 3 and 4, stem cell specific markers; Oct4, SSEA-1 and SSEA-3 were detected. In group 4, genes of primordial germ cell marker; VASA, premeiotic marker; Daz1and post-meiotic markers; c-Kit, Stra 8 were expressed. Daz1 was also expressed in group 3 rats to a significantly lower extent in comparison to group 4 rats. Spermatocytes and spermatids were detected in testicular tissue of group 4 rats. In conclusion, MSCs have potentials for in vitro transdifferentiation into germ cells and in vivo transdifferentiation into spermatids and spermatocytes.

Mohammed T. Abdel Aziz, Unit of Biochemistry and Molecular Biology Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo
Unit of Biochemistry and Molecular Biology
Taymour Mostafa, Andrology & Sexology, Faculty of Medicine, Cairo University, Cairo
Andrology Sexology
Hazem Atta, Unit of Biochemistry and Molecular Biology Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo
Medical Biochemistry Department
Soheir Asaad, Histology Department, Faculty of Medicine, Cairo University, Cairo
Histology Department
Hanan H. Fouad, Unit of Biochemistry and Molecular Biology Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo
Medical Biochemistry Department
Gamal Mohsen, Unit of Biochemistry and Molecular Biology Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo
Medical Biochemistry
Laila Rashed, Unit of Biochemistry and Molecular Biology Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo
Medical Biochemistry
Dina Sabry, Unit of Biochemistry and Molecular Biology Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo
Medical Biochemistry
Mohammed Abbas, Unit of Biochemistry and Molecular Biology Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo
Medical Biochemistry

Supporting Agencies

Unit of Biochemistry and Molecular Biology Faculty of Medicine Cairo University

Abdel Aziz, M. T., Mostafa, T., Atta, H., Asaad, S., Fouad, H. H., Mohsen, G., Rashed, L., Sabry, D., & Abbas, M. (2011). In vitro and in vivo lineage conversion of bone marrow stem cells into germ cells in experimental Azoospermia in rat. Stem Cell Studies, 1(1), e15. https://doi.org/10.4081/scs.2011.e15

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