Antitumor potential of Citrus limetta fruit peel in Ehrlich ascites carcinoma bearing Swiss albino mice

  • Sriparna Kundusen Department of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, India.
  • Asis Bala Department of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, India.
  • Biswakanth Kar Department of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, India.
  • Sanjib Bhattacharya Bengal School of Technology (a College of Pharmacy), Sugandha, Hooghly, West Bengal, India.
  • Upal K. Mazumder Department of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, India.
  • Malaya Gupta Department of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, India.
  • Pallab K. Haldar | pallab_haldar@rediffmail.com Department of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, India.

Abstract

Citrus limetta Risso (Rutaceae), commonly known as sweet lime in English and Mousambi in India, has been traditionally used for several medicinal purposes. This study explored the relationship between Citrus limetta fruit peel and its antitumor activity against Ehrlich ascites carcinoma (EAC) bearing mice. The antitumor activity of methanol extract of peel of Citrus limetta fruits (MECL) was evaluated against EAC cell line in Swiss albino mice. Twenty-four hours after intraperitoneal inoculation of tumor EAC cells in mice, MECL was administered at 200 and 400 mg/kg body weight i.p. daily for nine consecutive days. On the 10th day, half of the mice were sacrificed for the estimation of tumor growth (tumor volume, viable and non-viable tumor cell counts), and hematologic parameters (red blood cells, white blood cells and hemoglobin). The rest were kept alive for assessment of survival parameters, i.e. median survival time and percentage increase in life span of EAC bearing mice. Intraperitoneal administration of MECL at the doses of 200 and 400 mg/kg for nine days to the carcinoma induced mice demonstrated a significant (P<0.001) decrease in tumor volume, viable tumor cell count, tumor weight and a significant (P<0.001) improvement in hematological parameters and life span as compared to the EAC control mice. The present study establishes marked and dose dependant anti-tumor effect of C. limetta fruit peel against Ehrlich ascites carcinoma bearing Swiss mice.

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Published
2012-08-14
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Original Articles
Keywords:
antitumor, 5-fluorouracil, hematologic, median survival time
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How to Cite
Kundusen, S., Bala, A., Kar, B., Bhattacharya, S., Mazumder, U. K., Gupta, M., & Haldar, P. K. (2012). Antitumor potential of Citrus limetta fruit peel in Ehrlich ascites carcinoma bearing Swiss albino mice. Alternative Medicine Studies, 2(1), e10. https://doi.org/10.4081/ams.2012.e10