Cultural characteristics and antibiotic susceptibility pattern of Helicobacter Pylori isolated from dyspepsia patients

  • Bolanle A. Adeniyi | lawaltemitope8@gmail.com Department of Pharmaceutical Microbiology, University of Ibadan, Nigeria.
  • Temitope O. Lawal Department of Pharmaceutical Microbiology, University of Ibadan, Nigeria.
  • Jesse A. Otegbayo Department of Medicine, University of Ibadan, Nigeria.
  • Olayiwola A. Oluwasola Department of Pathology, University of Ibadan, Nigeria.
  • Georgina N. Odaibo Department of Virology, University of Ibadan, Nigeria.
  • Samuel O. Ola Department of Medicine, University of Ibadan, Nigeria.
  • Clement A. Okolo Department of Pathology, University of Ibadan, Nigeria.
  • Adegboyega Akere Department of Medicine, University of Ibadan, Nigeria.
  • Aderemi O. Kehinde Department of Medical Microbiology, University of Ibadan, Nigeria.

Abstract

Helicobacter pylori consist in a helical shaped Gram-negative bacterium, approximately 3 micrometers long with a diameter of approximately 0.5 micrometers. It has 4-6 flagella. It is microaerophilic and tests positive for oxidase, catalase and urease. With its flagella, the bacterium moves through the stomach lumen and drills into the mucus gel layer of the stomach. In humans, H. pylori have been associated with peptic ulcers, chronic gastritis, duodenitis and stomach cancer. It is widely believed that in the absence of treatment, H. pylori infection, once established in its gastric niche, persists for life. The aim of this research is to study the cultural characteristics and antibiotic susceptibility pattern of H. pylori strains isolated from southwest Nigeria. The cultural characteristics and antibiotic susceptibility pattern of Helicobacter pylori strains isolated from gastric mucosal antral biopsy specimens collected from 43 of 52 dyspepsia patients in the University College Hospital Ibadan, Oyo State, Nigeria, were determined using standard microbiological methods for Helicobacter pylori isolation. The 43 isolates were subjected to 23 different antibiotics and each of the antibiotics demonstrated a variable degree of activity against the isolates. Among the antibiotics to which the organism was most susceptible are: ofloxacin (30 μg) 100% activity, ciprofloxacin (5 μg) 97.67% activity, gentamicin (120 μg) 95.35 activity, amikacin (30 μg), kanamycin (30 μg) and chloramphenicol (30 μg) each 90.70% activity, clarithromycin (15 μg) 93.02, while the less active antibiotics are: augmentin (30 μg) 23.26% active, amoxycillin (25 μg) and metronidazole (50 μg) each 27.91% active and clindamycin (2 mg) 30.23% active. From the result of the antibiotic susceptibility pattern of the strains of the organism, 95.35% of the total isolates are multi drug resistant. Resistance was developed to, among others, augmentin (30 μg), amoxycillin (25 μg), metronidazole (50 μg) and clindamycin (2 mg).

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Published
2012-11-19
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Keywords:
Helicobacter pylori, cultural characteristics, antibiotics susceptibility pattern, multi drug resistance
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How to Cite
Adeniyi, B. A., Lawal, T. O., Otegbayo, J. A., Oluwasola, O. A., Odaibo, G. N., Ola, S. O., Okolo, C. A., Akere, A., & Kehinde, A. O. (2012). Cultural characteristics and antibiotic susceptibility pattern of Helicobacter Pylori isolated from dyspepsia patients. Gastroenterology Insights, 4(2), e21. https://doi.org/10.4081/gi.2012.e21