Tecchio, Andreini, Costantini, Zamò, Sabata, Aprili, Galavotti, Guardalben, and Benedetti: Haploidentical hematopoietic stem cell transplantation in a myelofibrosis patient with primary graft failure

Haploidentical hematopoietic stem cell transplantation in a myelofibrosis patient with primary graft failure




Competing interest statement

Conflict of interest: the authors declare no potential conflict of interest.

Abstract

The prognosis of patients affected by myelofibrosis (MF) is usually dismal and allogeneic hematopoietic stem cell transplantation (HSCT) remains the only cure. The number of HSCTs in MF patients has recently increased. However, a major obstacle is still represented by primary graft failure (PGF). Currently there are no definitive guidelines for the treatment of PGF and a second HSCT can be performed only when an allogeneic donor is rapidly available. Herein we report on a MF patient with PGF after an unrelated HSCT, who was rescued by a non-myeloablative, unmanipulated, haploidentical HSCT that resulted in persistent engraftment and bone-marrow fibrosis regression, but not in a long-term disease control. Based on this experience we briefly review the role of different conditioning regimens and hematopoietic stem cell sources in the setting of HSCT for MF patients with PGF. The role of haploidentical donors in MF patients lacking HLAmatched relatives is also discussed.

Introduction

Myelofibrosis (MF) is a pathological condition caused by the clonal proliferation of a pluripotent stem cell. It is characterized by a deregulated kinase signaling, and an abnormal release of cytokines, the latter being responsible for the induction of bonemarrow (BM) fibrosis.1 MF is associated with three main driver mutations namely the V617F mutation of the Janus Kinase 2 (JAK2) gene (in ~60% of cases), and mutations of the calreticulin (CARL) and the myeloproliferative leukemia protein (MPL) genes (in ~30% and 8% of cases, respectively). 1,2 MF can arise de novo as primary myelofibrosis (PMF) or following essential thrombocythemia or polycythemia vera. Clinical presentation of MF includes blood cytopenia, leukoerithroblastosis, extramedullary hematopoiesis, progressive splenomegaly, and systemic symptoms.3 In spite of the recent developments of targeted therapies (e.g., JAK1/2 inhibitors), the prognosis of MF patients is usually dismal and the allogeneic hematopoietic stem cell transplantation (HSCT) remains the only cure.3 Improvements in patient selection, conditioning strategies and timing, have recently contributed to increase the number of HSCTs in MF patients.4 However, one of the major obstacles for a successful HSCT is represented by primary graft failure (PGF), which has been reported in 2% to 25% of MF patients.1

PGF is caused by the absence of an initial cell donor engraftment and is defined as the persistence of severe neutropenia together with red blood cells and platelets transfusion dependence after the HSCT conditioning. It is associated with a considerable morbidity and mortality, mainly driven by severe infections. A number of factors contribute to PGF, including hematopoietic stem cell (HSC) source, conditioning regimen, HLA disparity, AB0 mismatches, and infections.5 In myeloproliferative neoplasms possible causes of PGF may also include a defective BM stroma, splenic consumption of infused HSCs and allo-immunization following multiple transfusions.5 In MF patients increased levels of plasma cytokines (mostly TNF-α) may also exert a cytotoxic activity against HSCs.1

Worthy of note BM fibrosis has not been clearly proven to correlate with PGF, but rather a consequence of an altered cytokine milieu able to reverse upon the replacement of the abnormal hematopoietic system.6 In the absence of definitive guidelines for the treatment of PGF patients may undergo a second HSCT, which can be performed using the same or another allogeneic donor when available.

Case Report

A 54-year-old woman was referred to our Institution on December 2012 with painful splenomegaly and systemic symptoms. Blood tests showed leukocytosis (24×109/L) and thrombocytopenia (97×109/L). The circulating CD34+ cells were 2.4%. The BM aspirate was not evaluable due to the poor cellularity, while the BM biopsy was consistent with PMF. The karyotype was normal and the molecular analysis revealed the presence of the JAK2 V617F mutation. The patient was diagnosed with an intermediate-1 PMF according to the International Prognostic Scoring System.7 She was first treated with hydroxyurea, then with ruxolitinib, which became available in our Institution starting from February 2013. In spite of an initial reduction of the spleen size and the remission of the systemic symptoms, on May 2014 ruxolitinib was withdrawn due to the reappearance of both painful splenomegaly and systemic symptoms, and the patient underwent splenectomy followed by treatment with hydroxyurea.

Although the Dynamic International Prognostic Scoring System (DIPSS) score was now intermediate-2,8 the HSCT was not performed because the only patient’s brother was HLA haploidentical and no unrelated donors were unavailable. A few months later, a 10/12 matched male donor (antigen mismatch in DPB1) was identified and a peripheral blood hematopoietic stem cell (PBSC) HSCT could be scheduled. Patient and donor differed in AB blood group systems and CMV serostatus (B+ vs A+, CMV IgG positive vs negative). The BM biopsy done before the HSCT was revealing that the patient’s BM had a diffuse and intense fibrosis (Figure 1).9 At that time the DIPSS score was still intermediate-2, and the HSCT-comorbidity index was low.10 The HSCT was finally administered on March 2015 with a myeloablative conditioning consisting of busulfan (16 mg/kg i.v.) and cyclophosphamide (120 mg/kg i.v.). The patient received 10.66×106/kg CD34+ cells, while the CD3+ cells infused were 24.47×106/kg. Graft-versus-host disease (GVHD) prophylaxis included rabbit antithymocyte globulins, cyclosporine and short course methotrexate. In spite of the high number of CD34+ cells infused, on day +28 the patient was diagnosed with PGF. At that time an empirical antibacterial and antimycotic therapy was being delivered due to a persistent fever and the CTscan evidence of a single excavated lesion of 5-6 cm on the upper lobe of the left lung in the absence of galactomannan positivity.

The urgent requirement for a new donor and the rapid worsening of the patient’s clinical condition (ECOG3) prompted us to schedule as a salvage treatment a PBSC HSCT from the haploidentical brother, who was AB0-mismatched (A+) and CMV IgG positive. Therefore, on day +37 from the first HSCT, the patient received 6×106/kg CD34+ and 32.6×107/kg CD3+ cells from the brother, following a conditioning regimen consisting of only fludarabine (30 mg/m2 day for 3 days). On day +3 after the haploidentical HSCT the patient underwent cyclophosphamide (50 mg/kg i.v.) and starting from day +4 cyclosporine and mycophenolate mofetil as GVHD prophylaxis. G-CSF was given from day +5 and it was stopped on day +13, when the engraftment was documented. Starting from the same time the patient presented a grade II skin GVHD that required steroid therapy until day +49. Following the haploidentical HSCT, the BM aspirate was performed on day +21, +90, and +180, showing a fulldonor chimerism by either fluorescence in situ hybridization with sex chromosomespecific DNA probes, and variable number tandem repeats analysis. Worthy of note, the BM biopsies performed on day +90 and +180 showed a progressive reduction of BM fibrosis as shown in Figure 1.

Importantly, the excavated lesion of the left lung was progressively decreasing and no GVHD was observed.

Unfortunately, on day +290 a relapse of disease was documented and the patient died of pulmonary infection few months later while in palliative treatment.

Discussion

The immediate availability of new donors for patients experiencing PGF is of critical importance and in this perspective the use of related haploidentical donors may offer obvious advantages. However, the new conditioning regimen together with the increased immunosuppression required to overcoming HLA disparity, may cause severe organ toxicities and infections. Recent reports have shown that haploidentical HSCT, either T-cell depleted,11,12 or unmanipulated,13-17 usually preceded by reduced intensity conditioning (RIC), can be safely and effectively administered to patients with PGF. However, the patients described in the aforementioned reports were mainly affected by acute or chronic myeloid leukemia, juvenile myelomonocytic leukemia, or myelodisplastic syndrome. 11-17

To the best of our knowledge, the successful administration of unmanipulated haploidentical HSCTs has been previously described only in two MF patients with a follow-up of 30 and 11.5 months respectively, who had reported a PGF after an unrelated HLA full-matched RIC-HSCT.14 In both cases the 1-day conditioning regimen was composed of 30 mg/m2 fludarabine, 2 g/m2 cyclophosphamide, 20 mg alemtuzumab i.v., and 200 cGy total body irradiation, based on the assumption that the first preparative regimen might had already suppressed the recipient’s immune systems. As for our patient, the conditioning scheme initially scheduled,18 actually included only fludarabine (30 mg/m2 for 3 days) given the poor patient’s clinical status and the lack of any residual hematopoiesis at the BM biopsy of day +28 (not shown). Moreover, in contrast to the original schedule,18 we infused PBSCs instead of BM, due to the need of a rapid engraftment. Interestingly, in agreement with a recent study demonstrating a better outcome for PGF patients receiving PBMCs as compared to other stem cell sources,19 most of the PGF patients who underwent a haploidentical HSCT also received PBMCs.13-17

Worthy of note, in our patient the engraftment paralleled the progressive regression of BM fibrosis (Figure 1), therefore confirming previous experiences evidencing that a high fibrosis grading is not a major obstacle to HSCT in MF patients, but instead a dynamic process largely dependent on the microenvironment.6

As for our patient, the haploidentical HSCT was able to overcome the PGF and to reduce the BM fibrosis. However it turned out to be ineffective against the long-term control of the disease, thus suggesting that the conditioning regimen delivered and/or the transplant-related immune-mediated effect, were not capable of eradicating the disease.

Conclusions

Overall, given the limits of a single experience, this clinical case indicates that the unmanipulated haploidentical HSCT may be effective in rescuing MF patients from PGF. In addition, the higher number of PGF observed in patients undergoing unrelated HSCT,19 together with the difficulties of delivering a new conditioning regimen, support recent data indicating that the haploidentical donor may be a reasonable first option for MF patients lacking a HLAmatched relative.20 We are aware that only prospective studies aimed at comparing the outcome of recipients of conventional versus alternative donors will definitively establish the role of haploidentical HSCT in MF patients.

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Figure 1.

Grading of bone-marrow fibrosis (MF) according to the WHO guidelines using the Gomori stain. MF was evaluated: A) before the unrelated allogeneic HSCT, B) before the haploidentical HSCT, C) on day +90 and D) on day +180 following the haploidentical HSCT. Final magnification 200×.

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Copyright (c) 2017 Cristina Tecchio, Angelo Andreini, Claudio Costantini, Alberto Zamò, Donata de Sabata, Fiorenza Aprili, Roberta Galavotti, Emanuele Guardalben, Fabio Benedetti

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