Nanoparticles containing siRNA to silence CD4 and CCR5 reduce expression of these receptors and inhibit HIV-1 infection in human female reproductive tract tissue explants

Human Immunodeficiency Virus-type 1 (HIV-1) binds to CD4 and CCR5 receptors on target cells in the human female reproductive tract. We sought to determine whether reducing levels of messenger RNA (mRNA) transcripts that encode these receptors in female reproductive tract cells could protect mucosal tissue explants from HIV-1 infection. Explants prepared from the endometrium, endocervix, and ectocervix of hysterectomy tissues from HIV-1 sero-negative women were exposed to nanoparticles containing CD4- and CCR5-specific short-interfering RNA (siRNA) sequences. Explants were then exposed two days later to HIV-1, and HIV-1 reverse transcripts were measured five days post-infection. Explants treated with nanoparticles containing CD4- and CCR5-specific siRNA showed reduced levels of CD4 and CCR5 transcripts, and significantly lower levels of HIV-1 reverse transcripts compared to those treated with an irrelevant siRNA. In female reproductive tract explants and in peripheral blood cell cultures, siRNA transfection induced the secretion of IFN-alpha (IFN-α), a potent antiviral cytokine. In female mice, murine-specific Cd4-siRNA nanoparticles instilled within the uterus significantly reduced murine Cd4 transcripts by day 3. Our findings demonstrate that siRNA nanoparticles reduce expression of HIV-1 infectivity receptors in human female reproductive tract tissues and also inhibit HIV-1 infection. Murine studies demonstrate that nanoparticles can penetrate the reproductive tract tissues in vivo and silence gene expression. The induction of IFN-α after siRNA transfection can potentially contribute to the antiviral effect. These findings support the therapeutic development of nanoparticles to deliver siRNA molecules to silence host cell receptors in the female reproductive tract as a novel microbicide to inhibit mucosal HIV-1 transmission.


Introduction
Women acquire HIV-1 infection after exposure to cell free or cell-associated virus in the female reproductive tract or the rectal mucosa. Viral particles or cell-associated virus in semen infect susceptible target cells in the underlying lamina propria. [1][2][3] The most likely targets for infection include macrophages, dendritic cells (DC), Langerhans cells and T cells. These cells express some or all of the receptors that permit viral binding, internalization, and infection, including CD4, CCR5, CXCR4 and DC-SIGN. Blocking the interaction between HIV-1 and infectivity receptors on target cells is an effective mechanism to inhibit HIV-1 infection. 4 This has been successfully demonstrated using antibodies, 5 soluble proteins, 6,7 and chemokines. [8][9][10] Some of these inhibitors are currently being tested pre-clinically as microbicidal agents to prevent HIV-1 sexual transmission. [11][12][13][14][15] However, the delivery of compounds that act on the cell surface are often less effective in mucosal tissues due to local conditions that degrade soluble proteins, and to physical barriers that prevent such molecules from penetrating the mucus covering and reaching cells in the lamina propria. Thus, the challenge exists to develop effective and non-toxic microbicidal compounds to interfere with the interaction between HIV-1 and its target cells in mucosal tissues.
An alternative approach to inhibit the interaction between HIV-1 and its target cell in the female reproductive tract is to reduce the cellular expression of receptors and co-receptors important in viral binding. Support for this approach comes from the observation that individuals who are heterozygous for the delta-32 CCR5 mutation are significantly less likely to become infected with HIV-1 after sexual exposure than are individuals who express the wild-type form of the receptor. 16,17 This indicates that even a partial reduction in receptor availability can significantly impact a person's susceptibility to acquiring sexually-transmitted HIV-1 infection. Reducing expression of cellular receptors in tissues can be achieved by the use of RNA interference (RNAi). RNAi uses small segments of complementary RNA called short-interfering RNA (siRNA) that are 20-25 nucleotides in length, to bind specific mRNA sequences within a cell. These complexes are then targeted for degradation by the formation of an RNA-induced silencing complex. siRNA sequences are highly specific and found to be efficacious in reducing expression of targeted genes. The use of RNAi is an attractive mechanism for reducing targeted gene expression in a therapeutic setting, is already in use clinically to treat including macular degeneration, 18 and is currently under development for metabolic diseases, 19 cancer, 20 and viral dis-eases including HIV-1. 21 Given the potential to specifically silence any gene of interest, siRNA offers several advantages over conventional drugs. To transfect the siRNA into cells, siRNA sequences are encapsulated into nanoparticles using various molecules that are readily endocytosed by phagocytic cells. 22 Earlier studies using RNAi to inhibit HIV-1 infection employed siRNA sequences that silenced expression of viral sequences including Rev, Nef and Tat. [23][24][25] Silencing cellular genes important for HIV-1 infection has also been explored. Martinez co-receptors in tissue culture cells. The inhibitory effect of gene silencing was detected 48 h after transfection and reduced receptor expression in over one-half of receptor-positive cells. 26 The suppression of receptor expression effectively blocked the acute infection of CXCR4 + U87 cells by X4 (HIV-1NL4-3) and CCR5 + U87 cells by R5 (HIV-1BaL) HIV-1 strains. Arrighi, et al. used short hairpin RNA (shRNA)-expressing lentiviral vectors to inhibit the attachment of HIV-1 gp120 to DC-SIGN, as well as to inhibit the transfer of HIV-1 to target cells in trans. 27 RNAi-mediated CCR5 silencing was achieved in vivo in a humanized murine model by incorporating integrin-targeting sequences into liposome particles that encapsulated CCR5-specific siRNA. 28 These nanoparticles were targeted specifically to leukocytes by binding to the integrin-binding receptor, LFA-1, present on these cells. Animals who received an intravenous inoculation of these nanoparticles prior to intraperitoneal challenge with HIV-1 demonstrated a resistance to infection as determined by reductions in plasma viral load and maintenance of CD4 counts compared to untreated animals. The potential to target siRNA specifically to T cells in vivo was reported by Kumar,et al. 29 siRNA to CCR5 was encapsulated in nanoparticles and these nanoparticles were complexed to an antibody with specificity for the pan T cell marker, CD7, to target siRNA to T cells in vivo. This modified siRNA was infused into human CD34 + progenitor cellreconstituted animals together with antibody to CCR5, prior to the intravenous infusion of HIV-1. The combination of an antibody to CCR5 to block HIV-1 binding to CCR5 together with siRNA to down-regulate CCR5 expression suppressed HIV-1 infection and prevented CD4 + T cell loss in this animal model. 29 To assess whether this technology could be useful clinically in patients already infected with HIV-1, mice were instead reconstituted with peripheral blood cells from an HIV-infected patient who was on anti-viral therapy and who had an undetectable viral load. The infusion of CCR5silencing siRNA nanoparticles targeted to T cells with anti-CD7 antibody maintained levels of CD4 + cells in the bloodstream and kept viral load levels in the undetectable range, compared to mice that received an irrelevant siRNA. Nanoparticle delivery has also been explored as an approach to deliver microbicidal compounds and other anti-viral therapeutics to mucosal tissues of the human female reproductive tract. 30 The encapsulation of bioactive compounds within nanoparticles not only protects these agents from enzymatic destruction by the acidic pH and enzymes of the vagina, but allows rapid and targeted uptake by phagocytic cells in the lamina propria. 31 Thus, the development of nanoparticle transfection for the delivery of siRNA, bioactive compounds, and vaccine antigens to the mucosal tissues of the female reproductive tract could significantly advance therapeutic approaches to combat HIV-1 infection in these tissues. As HIV-1 vaccine trials have failed to produce a protective vaccine, the development of microbicidal compounds that can protect women from sexuallytransmitted infection have received considerably more attention in recent years. 12 In this study, we sought to determine the efficacy of RNAi to inhibit HIV-1 infection in human female reproductive tract tissue explants. We used a novel primary tissue explant model 32 in which explants from the human female reproductive tract were transfected with nanoparticles to deliver CD4 and CCR5-specific siRNA, and then challenged with HIV-1. The small size of these nanoparticles promoted endocytosis by phagocytic cells in the explants, thus transfecting target cells with the siRNA. Our findings demonstrate significant inhibition of HIV-1 infection in explants prepared from the endometrium, endocervix and ectocervix obtained from HIV-1 seronegative women undergoing hysterectomy for benign conditions. To determine the ability of siRNA nanoparticles to penetrate mucosal tissues in vivo, we assessed their efficacy to silence murine Cd4 after instillation into the reproductive tract of female mice. Significant silencing of murine Cd4 expression was detected in uterine tissues on day 3 after a single application of siRNA nanoparticles. In addition, we demonstrate that siRNA transfection induced the expression of IFN-α, a potent antiviral cytokine that may contribute to the anti-HIV effect. These findings suggest that RNAi to silence cellular genes important for HIV-1 infection in mucosal tissues can be developed as a potential therapeutic approach to inhibit HIV-1 transmission resulting from heterosexual exposure.

Female reproductive tract tissues
Female reproductive tract tissues were obtained from HIV-seronegative women undergoing hysterectomy at Dartmouth-Hitchcock Medical Center for benign conditions including fibroids, prolapse and dysmenorrhea. Informed consent was obtained prior to surgery. The age, diagnosis, menstrual cycle stage and hormonal therapy at the time of hysterectomy of each subject are shown in Table 1. Tissues were dissected free of underlying musculature within 4 hours of surgery, and the mucosal layers comprising both the epithelium and lamina propria were cut into 3 mm 3 sections. Two sections from each tissue site were placed into wells of a 48-well plate and cultured in 0.5 mL of Leibovitz's medium (L-15, Invitrogen, Carlsbad, CA, USA) containing 10% heat-inactivated FBS, 2 mM glutamine, 50 units/mL penicillin and 50 µg/mL streptomycin (Invitrogen), at 37°C, 5% CO2 in a humidified atmosphere.
siRNA transfection of tissue explants siRNA specific for human CD4 (s225098), CCR5 (s3211), or an irrelevant sequence (4390843), were obtained from Applied Biosystems/Ambion (Carlsbad, CA, USA). The encapsulating/transfection reagent INTERFER- Unknown/none *Estrace cream is a synthetic form of topically applied estrogen commonly used to treat vaginal atrophy; Lupron depot is a gonadotropinreleasing hormone sometimes used before hysterectomy in women with menorrhagia and anemia to prevent further bleeding prior to surgery; Alesse-28 is a synthetic progesterone and estradiol combination used as a contraceptive.

Article
in was obtained from Genesee Scientific (San Diego, CA, USA). INTERFERin carries a net positive charge, and when combined with the negatively charged siRNA molecules under vigorous vortexing forms nanoparticles that encapsulate the siRNA. Nanoparticles prepared in this manner and then stained with uranyl acetate and visualized by transmission electron microscopy are between 45 and 60 nm in diameter. 33 Nanoparticles were prepared by vortexing a solution containing 6 pmoles of siRNA, 2.4 µL of INTERFERin, and 100 µL of serum free media, followed by 15 min incubation at room temperature. This nanoparticle suspension was added to each well of tissue explants or peripheral blood mononuclear cells (PBMC) to give a final concentration of 20 nM siRNA. Untreated tissue explants received only 100 µL of the serum-free media per well. Twenty-four hours later, wells containing tissue explants were supplemented with 0.5 mL of fresh media ( Figure 1). The following day, tissue explants were washed extensively and suspended in fresh media.

HIV-1 infection of tissue explants
Some of the issue explants were exposed to cell-free HIV-1Ba-L at a final virus concentration of 200 TCID50/well 48 h after siRNA transfection ( Figure 1). This concentration of virus was previously determined to be optimal for infecting tissue explants (data not shown). Viral stocks were treated with 10 units/mL of RNase-free DNase-I prior to use to destroy any contaminating genomic DNA. After overnight incubation with virus at 37°C, the female reproductive tract tissue sections were washed three times to eliminate non-internalized virus, and replenished in L15 medium.

Nucleic acid isolation
RNA was isolated from uninfected human tissue explants treated with siRNA nanoparticles to assess the magnitude of CCR5 and CD4 gene silencing. The replicate explants, plus any cells that migrated out of the tissue explant into the surrounding media (migratory cells), were collectively harvested after siRNA transfection and stored in RNAlater (Ambion, Austin, TX, USA) at -80o C until processing. Endocervix and endometrial tissues were harvested on days 3 and 6 post-silencing, whereas ectocervix tissue sections were harvested and assessed more frequently at days 1, 2, 3, 4, 6 and 8 post-silencing. Tissues were thoroughly homogenized in RLTplus lysis buffer (Qiagen, Valencia, CA, USA) with a scintered glass homogenizer (Kontes, Vineland, NJ, USA). The lysate was subjected to RNA isolation using an RNeasy-Plus kit (Qiagen).
DNA was isolated from tissue explants and from migratory cells in culture on day 5 after infection (corresponding to day 8 post-siRNA transfection) using lysis buffer from Gentra PureGene Tissue Kit (catalog 158667, Qiagen) and columns from QIAamp DNA extraction kit (catalog 51306, Qiagen). DNA was analyzed by real-time PCR for HIV-1 reverse transcripts.

Murine siRNA studies
All animal components of the research protocols (ACORP) were approved and supervised by our Institutional Animal Care and Use Committee (IACUC). Six to 8 week old female Balb/C mice were obtained from Harlan Laboratories (South Easton, MA, USA). Nanoparticles for in vivo use were prepared by vortexing siRNA in 5% glucose/95% water with in vivo Jet PEI (GeneSee Scientific) at an N/P ratio of 8. Forty µL of the suspension containing 40 µM of Cd4 specific siRNA (s63657, Applied Biosystems/Ambion) or an irrelevant siRNA (ss20212, Applied Biosystems/Ambion) was instilled separately into each uterine horn by loading a pipet tip with the solution, and inserting it atraumatically into the vaginal canal and past the cervical os, directing the solution first into one uterine horn, then reinserting a second application of an identical volume into the other uterine horn. Mice were anesthetized with inhalation isofluorane immediately prior to and during the instillation, and were kept anesthetized and in a head down position for 5 minutes afterwards to prevent the solution from leaking out of the vaginal canal. Two to four mice from each experimental group (Cd4 or irrelevant siRNA) were euthanized by CO2 inhalation on days 1, 2, 3, and 5 post-siRNA instillation, and reproductive Article Figure 1. Timeline of the experimental protocols using human female reproductive tract explants. Samples of female reproductive tract tissues were received, dissected, and placed into culture on day 0, the same day they were transfected with nanoparticles containing siRNA. On day 2, the explants were thoroughly washed to remove residual siRNA nanoparticles, and some sections were exposed to cell-free HIV-1BaL. On day 3, the HIVexposed explants were again thoroughly washed to remove any unincorporated HIV-1. CCR5 and CD4 mRNA levels were measured on uninfected ectocervical explants on days 1, 2, 3, 4, 6, and 8, and only on days 3 and 6 in explants from endometrium and endocervix. HIV-1 DNA was measured on the HIV-1 exposed explant cultures on day 8. organs (vagina plus cervix, uterine horns) were removed and stored in RNAlater (Qiagen, Valencia, CA, USA) at -80°C. The tissues were then thawed and homogenized, and RNA isolated as described. 35 Real time PCR was used to quantitate Cd4 using murine specific Cd4 primers (5'-TGCAAACACAAAAAGGGTAAA-3' and 5'-TACGACCAGAGGCATACAGGGACAG-3'), and normalized to murine β-actin (sense primer 5'-ACCAACTGGGACGACATGGAGAAGA-3' and anti-sense primer 5'-TACGACCAGGAG-GCATACAGGGACAG-3').

PBMC isolation
Peripheral blood was obtained after informed consent from normal donors, and the mononuclear cell fraction isolated by Ficoll-Paque (Amersham, Piscataway, NJ, USA) as described. 35 PBMC were suspended to 2¥10 6 cells/mL in RPMI-1640 supplemented with 10% heat-inactivated FBS, 2 mM glutamine, 50 units/mL penicillin and 50 µg/mL streptomycin (GIBCO). Two million cells were added to wells of a six-well plate prior to transfection with siRNA nanoparticles.

IFN-α secretion from siRNA-treated PBMC and tissue explants
Supernatants were collected from PBMC exposed to siRNA nanoparticles, or from PBMC left untreated, immediately prior to siRNA transfection, and again at 4, 24, 48 and 96 h post-transfection. Levels of IFN-α in the supernatant were measured by ELISA (R and D Systems, Minneapolis, MN, catalog #41105-1, IFN-α Multi-Subtype ELISA Kit).
As the levels of secreted IFN-α from tissue explants were below the level of detection of the IFN-α ELISA kit, we quantified expression of IFN-α from ECX tissue sections by realtime PCR. ECX tissues were chosen for study because this tissue type has the highest concentration of leukocytes compared to other sites within the female reproductive tract. 32 RNA isolated from siRNA-treated ECX tissue explants were subjected to stringent removal of contaminating DNA prior to amplification. IFN-α transcripts were amplified with the sense primer (5'-GCA CCG AAC TCT ACC AGC AGC-3') and anti-sense primer (5'-TCT GAC AAC CTC CCA GGC ACA-3'). These primers amplify a product that is 179 base pairs in size. 36

Statistical analysis
Analysis of datasets comparing two groups was performed by students' T-test, and those comparing multiple groups were performed by ANOVA, and were considered statistically significant at P<0.05.

Results
siRNA silences CD4 and CCR5 expression in female reproductive tract tissue explants after a single siRNA exposure CD4 and CCR5 expression was measured in endometrial (EM) and endocervical (CX) explants on days 3 and 6 post-siRNA transfection, and on ectocervical (ECX) explants on days at 3, 4, 6 and 8 post-siRNA transfection. For each subject, expression levels from CD4 and CCR5-specific siRNA-treated explants were normalized to sections treated with irrelevant siRNA to control for the effects of nanoparticle transfection on cells. We detected significant reductions in CD4 transcript levels in EM and ECX, but not in CX, in response to the combination of CD4 and CCR5 specific siRNA (Figure 2A). For example, CD4 expression was suppressed by approximately 45% in explants from the EM and ECX on day 6, and even more strongly suppressed (approximately 60%) in ECX on day 4 (Figure 2A). In contrast, CCR5 expression showed a different pattern. CCR5 was suppressed by specific siRNA on day 3 in the CX but this suppression was not sustained out to day 6. In the ECX, CCR5 was not suppressed until day 4 but its suppression was sustained throughout the 8 days of culture ( Figure 2B) reaching a maximum suppression of approximately 85% on day 6 compared to the irrelevant siRNA control. EM was refractory to suppression of CCR5 on both day 3 and 6 ( Figure 2B).

Time course of siRNA silencing of CD4 and CCR5 in ectocervical explants
We performed a detailed time course of CD4 and CCR5 transcript levels in ectocervical explants from a single subject to more closely assess comparisons among untreated, irrelevant siRNA and specific siRNA treatment on transcript levels. CD4 transcript levels in explants left untreated showed a drop over the first 3 days of culture and then a leveling off throughout the remainder of the culture period ( Figure 3A). Explants treated with an irrelevant siRNA nanoparticle showed a similar initial decrease in CD4 transcript levels and fluctuations in levels from days 4 through 8 of culture. In contrast, explants treated with nanoparticles containing the combination of CD4-and CCR5-siRNA showed a sustained suppression of CD4 transcript levels throughout the 8-day culture period ( Figure 3A). Analyses of CCR5 transcript levels indicate that in the untreated explants, there is a similar initial decrease in transcript levels in the first 3 days of culture, with a leveling off throughout the remainder of the culture period ( Figure 3B). Explants treated with an irrelevant siRNA showed a marked and reproducible increase in CCR5 transcript levels on day 4 post-transfection. This increase was followed by a down-regulation on days 6 and 8. In contrast, explants treated with the combination of CD4-and CCR5-siRNAs demonstrated a reduced level of CCR5 transcripts starting on day 3 when compared to both the untreated and irrelevant siRNA-treated explants ( Figure 3B). The receptor-specific siRNAs ablated any nonspecific stimulatory effects on both CD4 and CCR5 expression observed with the irrelevant siRNA containing nanoparticles.

HIV-1 infection is reduced in female reproductive tract explants silenced for CD4 and CCR5
Female reproductive tract explants left untreated, or transfected with nanoparticles containing either an irrelevant siRNA or the combination of siRNAs to CD4 and CCR5, were evaluated for HIV-1 reverse transcripts on day 8 of culture (day 5 post-infection). HIV-1 DNA levels were normalized to those observed in the irrelevant siRNA nanoparticle-treated explants. We observed a significant inhibition of HIV-1 reverse transcripts in all three tissue types (EM, CX and ECX) following transfection with specific siRNAs, compared to those explants treated with the irrelevant siRNA ( Figure 4). CX explants treated with the combination of CD4-and CCR5-specific siRNAs showed only a modest inhibition of HIV-1 infection (approximately 40%), whereas ECX explants had a very robust inhibition (approximately 80%) following CD4-and CCR5-siRNA treatment. For both the CX and ECX, HIV-1 infection in explants left untreated was approximately equivalent to the irrelevant siRNA treated explants. In contrast, HIV-1 infection in the untreated EM explants showed lower levels of HIV-1 reverse transcripts compared to those EM explants treated with the irrelevant siRNA nanoparticles. However, even in the EM, HIV-1 infection was significantly reduced in explants treated with nanoparticles containing the combination of CD4-and CCR5-siRNAs when compared to those treated with the irrelevant siRNA. In contrast, in the CX and ECX, treatment with the irrelevant siRNA nanoparticles did not increase HIV-1 transcript levels compared to the untreated explants. Thus, we observed a significant decrease in HIV-1 transcripts in all explants treated with nanoparticles containing the combination of receptor specific-siRNAs compared to the irrelevant siRNA (Figure 4).

siRNA nanoparticles silence murine Cd4 in reproductive tract tissues in vivo
To determine the efficacy of RNA interference using siRNA nanoparticles in an intact and functioning reproductive tract, nanoparticles containing murine Cd4-specific or irrelevant siRNA were instilled directly into both uterine horns of female mice. Uterine tissues analyzed on day 3 post-Cd4 siRNA nanoparticle instillation showed a significant reduction in murine Cd4 expression when compared to tissues from the irrelevant siRNA-treated control mice (P<0.05, Figure 5A) although this silencing effect was not observed at day 5. To determine whether the reduction in Cd4 transcript levels may have resulted from fewer numbers of murine leukocytes in the uterus of Cd4 siRNA-treated mice, we analyzed murine Cd45 transcript levels in these tissues. We observed no significant alterations in Cd45 transcript levels in uterine tissues obtained from mice treated with the Cd4-siRNA nanoparticles compared to those treated with the irrelevant siRNA control. Using real time PCR to quantify Cd4 and Cd45 transcript levels, no significant reductions were detected in the vaginal/cervical tissues that were downstream of the nanoparticle instillation site (data not shown). Real time PCR data were normalized to murine β-actin.

PBMC and tissue explants treated with siRNA nanoparticles secrete IFN-α
To determine mechanisms other than gene silencing that could contribute to the antiviral effect of siRNA transfection of tissue explants, we measured levels of IFN-α released into the culture supernatant from PBMC from cells left untreated, or treated with nanoparticles containing either an irrelevant siRNA or the combination of CD4-and CCR5-specific siRNAs. IFN-α secretion was detected using ELISA as early as 24 hours post-transfection in cells treated with nanoparticles containing either an irrelevant siRNA or CD4-plus CCR5-specific siRNAs. IFN-α was detected in the culture supernatant throughout the 72 hr culture period ( Figure 6A). No IFN-α secretion was detected at any time-point from the untreated PBMC.
Secretion of IFN-α by tissue explants was too low to detect by ELISA, so real-time PCR was used to measure expression of IFN-α transcripts in ectocervical explants. As shown in Figure 6B, we observed significant levels of IFN-α transcripts in ECX tissues treated with nanoparticles containing either irrelevant siRNA or the combination of CD4-and CCR5-siRNAs by day 2 after transfection, and this effect was sustained to day 4 ( Figure 6B). In both the PBMC cultures as well as the tissue explants, induction of IFN-α protein (PBMC) or its transcript (explants) was greater in cultures treated with nanoparticles containing the combination of CD4-and CCR5-siRNA as compared to the irrelevant siRNA.

Discussion
The female reproductive tract tissue explant model has been invaluable for studies of mucosal immunity, HIV-1 infection, and microbicidal efficacy. Despite limitations to using human female reproductive tract explants in these types of studies, 37 the availability of tissues from clinically-defined subjects free of gynecological malignancy has allowed rapid advances in our understanding of the mechanisms involved in the early events in HIV-1 infection at this site, and in ways to block or otherwise inhibit viral infection either with vaccines or microbicides. We previously demonstrated that tissue explants prepared from the female reproductive tract of seronegative women could be infected with HIV-1, and that the level of infection among female reproductive tract sites correlated with expression of the pan-leukocyte marker, CD45. 32 In this study we exposed female reproductive tract explants to siRNA to silence CD4 and CCR5 for 48 h prior to the addition of HIV-1 to determine the efficacy of gene silencing to protect tissue explants from HIV-1 infection. We then measured the degree of gene silencing and of inhibition of HIV-1 reverse transcription on subsequent days of culture. Control tissue explants were treated with nanoparticles containing irrelevant siRNA to control for any effects on susceptibility to HIV-1 infection induced by nanoparticle uptake. Such effects have been reported and include cellular activation, effects on cellular viability, or bystander effects on the expression of non-targeted gene products. [38][39][40] We found that silencing expression of CD4 and CCR5 in these explants using nanoparticles to deliver siRNA reduced expression of targeted gene products and inhibited HIV-1 reverse transcription. Thus, it is likely that reducing expression of receptors required by HIV-1 for infection can result in an inhibition of HIV-1 infection in mucosal tissues, including the female reproductive tract.
In human female reproductive tract Bars with no asterisks did not reach statistical significance at the 95% confidence level.

Article
explants, we observed that the kinetics and magnitude of gene silencing after a single application of siRNA nanoparticles varied among the three tissue sites (EM, CX and ECX). This finding is likely due in part to differences among tissue sites in the number and density of leukocytes present in the lamina propria. In our previous study, we determined that the ECX has the highest level of CD45 expression in the female reproductive tract, indicating an enrichment of leukocytes at this site. 32 Moreover, we have found that the expression of CD4, CCR5, and CXCR4 varied in the ECX and in the EM on both the stromal leukocyte population as well as on epithelial cells, and the numbers and distribution of receptor-positive leukocytes also varied throughout the menstrual cycle, indicating hormonal regulation of receptor expression and leukocyte trafficking. 41,42 Thus, the varied findings among tissue sites is likely due to multiple effects including the density of receptor-positive cells, or to donor variation in age, menstrual cycle stage and/or clinical conditions. In addition, the efficacy of siRNA delivery via nanoparticles may be impacted by the degree of mucus within the female reproductive tract, or to other physical barriers such as the thickness or the type of epithelium. Nanoparticles must penetrate through the mucus layer and reach target cells in the lamina propria in order to bind to cells and deliver the siRNA molecule. Moreover, columnar epithelium lines the tissues of the upper reproductive tract (CX and EM), and nanoparticles may penetrate through this single cell layer more easily than the stratified epithelial lining of the lower reproductive tract (ECX). Thus, the combined effects of target cell density, thickness of the mucus layer and type of epithelium are likely to contribute to the varied efficacy of RNA interference in the female reproductive tract. Although we did not measure the penetration or distribution of the nanoparticles into the tissue explants, we have determined using isolated populations from the peripheral blood that nanoparticles are selectively engulfed by monocytes and not by lymphocytes (data not shown). This may also be the case in tissues, and may contribute to the incomplete suppression of CD4 and CCR5 transcript levels, as these receptors are also expressed by lymphocytes in the lamina propria. Nonetheless, our findings indicate that cells within tissue explants do take up siRNA delivered within nanoparticles, and that the siRNA can effectively silence gene expression.
To determine if nanoparticles containing siRNA could penetrate mucosal tissues in vivo, we tested the efficacy of these particles to silence expression of Cd4 in the murine female reproductive tract. A single application of nanoparticles containing murine Cd4-specific siRNA instilled directly into both uterine horns significantly suppressed Cd4 expression only on day 3 post-transfection. We did not observe significant silencing prior to day 3, and the effect was short lived in that the effect was largely gone by day 5. This is likely due to the time required for penetration of nanoparticles in vivo, as well as to efficacy of nanoparticle uptake in receptor-positive cells. A more thorough assessment to identify the cell lineages that take up nanoparticles in vivo along with measurements of efficacy of gene silencing are currently underway and should contribute to dosage and dosing frequency for optimal gene silencing in vivo.
Interestingly, inhibition of HIV-1 reverse transcription by siRNA was most effective in the ECX, a female reproductive tract site that has a stratified epithelium and mucus secretion. This finding suggests that there may be a greater density of HIV-1 susceptible macrophages at this site, as macrophages readily endocytose nanoparticles. In all three tissue types, the use of receptor-specific siRNAs significantly inhibited HIV-1 transcription when compared to explants treated with an irrelevant siRNA nanoparticle. In the CX Article Figure 5. Nanoparticles containing siRNA to murine Cd4 reduce Cd4 expression but not Cd45 expression in murine uterine tissues. Nanoparticles containing siRNA to an irrelevant sequence or to murine Cd4 (10 µg/mouse) were instilled directly into each uterine horn. On days 1, 2, 3 and 5, the mice were euthanized and uterine tissues assessed by real-time PCR for Cd4 expression (Panel A) or for Cd45 expression (Panel B) in triplicate and normalized to murine β-actin expression. Average relative expression of Cd4 or Cd45 was compared between groups of mice treated with the irrelevant siRNA control (white bars) or Cd4-specific siRNA (grey bars). Two mice are represented in each group for days one and two and four mice are represented in each group for days 3 and 5. Error bars show the standard deviation of the means among the mice in each group. A statistically significant difference (as denoted by a single asterisk) between the irrelevant and Cd4-specific siRNA treated groups was observed on day 3 (P<0.05). Bars with no asterisk did not reach statistical significance at the 95% confidence level. and ECX, we observed no significant differences in the levels of HIV-1 reverse transcripts between explants that were left untreated and those treated with irrelevant siRNA nanoparticles. This was not the case in the EM, however, in which lower levels of HIV-1 reverse transcripts were measured in untreated explants compared to those treated with the irrelevant siRNA. One possible explanation for this finding is that exposure to siRNA containing nanoparticles enhances HIV-1 infectivity in the EM. Whether this results from differences in responsiveness to RNA molecules by innate immune cells at this normally sterile site, or to other off-target effects of nanoparticle transfection, is not known at this time. These findings do, however, show that the complete silencing of receptor expression is not necessary to inhibit HIV-1 infection. Alternatively, gene silencing of CD4 and CCR5 may inhibit the spread of HIV-1 infection in the tissues, rather than inhibit the initial infectivity event.
Although we did not correlate the kinetics of CD4 and CCR5 transcript inhibition with the concomitant reduction in surface expression, it is likely that the uptake of nanoparticles containing siRNA serves to inhibit HIV-1 infection by mechanisms other than receptor suppression.
To determine whether other mechanisms may contribute to inhibiting HIV-1 infection in the female reproductive tract explants, we measured the release of IFN-α, a potent antiviral cytokine, from PBMCs treated with siRNA nanoparticles. We chose to assess IFN-α production from nanoparticle-exposed cells for several reasons. Toll-like receptors (TLR)-7, 8 and 9 that belong to a class of innate immune receptors release anti-viral cytokines including IFN-α/β and λ upon activation. 43 These TLR are located in endosomes of phagocytic cells, and bind double-and single-stranded nucleic acids characteristic of viral and bacterial pathogens. As siRNA-encapsulated nanoparticles are taken up by cells in endocytic vesicles, it is likely that activation of these TLR would occur by binding to siRNA, leading to inflammatory cytokine production. 33,[44][45][46][47] Moreover, IFN-α has been associated with inhibiting HIV-1 replication in vitro, and with persistent HIV-1 infection in vivo. 48,49 Thus, the potential for siRNA-nanoparticles to both silence gene expression and activate innate immune responses could serve to enhance protection from HIV-1 infection in the female reproductive tract. We found that IFN-α was released from PBMC within 24 hours of siRNA transfection, and that levels of IFN-α were detectable in culture supernatants throughout the 4 day culture. Moreover, irrelevant siRNA also induced the release of IFN-α, although not to levels observed with receptor-specific siRNAs. No IFN-α was detected from untreated cells indicating a direct correlation between siRNA transfection and induction of this cytokine. Although we did not test whether the levels of secreted IFN-α were high enough to protect cells from HIV-1, it is possible that inflammatory responses to siRNA could contribute to the inhibition of HIV-1 infection in the female reproductive tract. Using real-time PCR, we found a similar induction of IFN-α transcription in ECX explants after siRNA treatment, but not until day 2 post-transfection. As with the PBMC cultures, both irrelevant and recep-tor-specific siRNA nanoparticles induced IFNα transcripts. Further studies are needed to determine the various mechanism(s) that are likely to contribute to HIV-1 inhibition of infection in female reproductive tract explants following siRNA transfection with nanoparticles.
In sum, our findings indicate that transfection of female reproductive tract tissue explants with siRNA-encapsulated nanoparticles results in a reduction of targeted gene expression and in inhibition of HIV-1 infection.  10 nM each, black bars). Levels are expressed as mean IFN-α transcripts relative to that observed at culture initiation (day 0). Error bars the show standard deviation of the means. Asterisks (*) denote statistically significant differences (P<0.05) between explants treated with siRNA as compared to untreated explants. Bars with no asterisk did not reach statistical significance at the 95% confidence level. This is an important finding because the use of siRNA has been effective in treating ocular diseases 18 and nanoparticles have been determined to be safe for mucosal tissue delivery Moreover, the use of nanoparticles for microbicidal delivery to both the female reproductive tract and the rectal mucosa indicates the potential for this approach to target primary HIV-1 infection in mucosal tissues. 12,[50][51][52] The potential to knock down gene expression within the female reproductive tract and induce protection against a sexually transmitted disease could be developed not only for HIV-1, but also for other pathogens that utilize cell-specific receptors for infection. Thus, it is possible to engineer nanoparticles for the delivery of siRNA, either alone or in combination with anti-HIV drugs, to achieve protection against sexual HIV-1 infection. Such nanoparticles could be manufactured in either a gel, film or ring formulation, so that they are stable upon storage, easy to apply, and can be designed to be timed-released and thus coitally-independent. 53,54 Nanoparticles can be designed to have optimal bio-distribution and residence time in mucosal tissues such that delivery of the bioactive components occurs throughout the lower reproductive tract (vaginal mucosa and cervix) at a continuous and stable rate. It is highly likely that nanoparticle-based microbicides can be designed with controlled architecture and surface chemistry that would retain efficacy in the mucosal tissues and allow delivery of active compounds without toxicity or damage to the epithelium. Such nanoparticle-based microbicides could provide HIV-1 protection for women until an effective HIV vaccine is developed.