Unusual 5'-regulatory structure and regulation of the murine Mlc1 gene: Lack of promoter-specific functional elements

  • Darja Henseler University of Trier, Institute of Psychobiology, Department of Neurobehavioral Genetics, Germany.
  • Jonathan D. Turner Laboratoire National de Santé, Luxembourg, Centre de Recherche Public-Santé, Institute of Immunology, Luxembourg.
  • Matthias Eckhardt University of Bonn, Institute of Biochemistry and Molecular Biology, Germany.
  • Maaike van der Mark Leiden University, LACDR/LUMC, Division of Medical Pharmacology, Netherlands.
  • Yanina Revsin Leiden University, LACDR/LUMC, Division of Medical Pharmacology, Netherlands.
  • Michelle K. Lin University of Trier, Institute of Psychobiology, Department of Neurobehavioral Genetics, Germany.
  • Thorsten Kranz University of Trier, Institute of Psychobiology, Department of Neurobehavioral Genetics, Germany.
  • Claude Muller Laboratoire National de Santé, Luxembourg, Centre de Recherche Public-Santé, Institute of Immunology, Luxembourg.
  • Jobst Meyer | meyerjo@uni-trier.de University of Trier, Institute of Psychobiology, Department of Neurobehavioral Genetics, Germany.

Abstract

The MLC1 gene is involved in an autosomal recessive neurological disorder, megalencephalic leucoencephalopathy with subcortical cysts (MLC), which is characterized by macrocephaly during the first year of life and swollen white matter (leucoencephaly). Variants of MLC1 have also been associated with psychiatric disorders such as schizophrenia, major depression and bipolar disorder. Currently, little is known about the encoded protein (MLC1). Judging from its similarity to other known proteins, it may serve as a trans-membrane transporter. However, the function of the encoded protein and its gene regulation has not been investigated successfully so far. We investigated the 5’ region of the murine Mlc1 with respect to regulatory elements for gene expression. A promoter search and an in silico analysis were conducted. Luciferase reporter gene constructs with potential promoter regions were created to study promoter activity in vitro. We found two alternative first exons for the murine Mlc1 but were not able to detect any promoter activity for the investigated reporter gene constructs in different cell lines, thus pointing to the presence of essential cis-acting elements far outside of the region. In silico analysis indicated an uncommon promoter structure for Mlc1, with CCAAT-boxes representing the only noticeable elements.

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Author Biography

Jobst Meyer, University of Trier, Institute of Psychobiology, Department of Neurobehavioral Genetics
Head of the department of Neurobehavioral Genetics
Published
2011-10-22
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Articles
Keywords:
Mlc1, schizophrenia, Pomp pseudogene, promoter regulation
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How to Cite
Henseler, D., Turner, J. D., Eckhardt, M., van der Mark, M., Revsin, Y., Lin, M. K., Kranz, T., Muller, C., & Meyer, J. (2011). Unusual 5’-regulatory structure and regulation of the murine Mlc1 gene: Lack of promoter-specific functional elements. Journal of Nucleic Acids Investigation, 2(1), e11. https://doi.org/10.4081/jnai.2011.2314