Standardization of lyophilization medium for Streptococcus thermophilus subjected to viability escalation on freeze drying

  • Rohit Sharma | accessrohit25@gmail.com Microbial Biotechnology Laboratory, Tropilite Foods Pvt. Ltd., Davars Campus, Gwalior; Department of Post Graduate Studies and Research in Biological Sciences, Rani Durgavati Vishwavidyalaya, Jabalpur, India.
  • Bhagwan S. Sanodiya Microbial Biotechnology Laboratory, Tropilite Foods Pvt. Ltd., Davars Campus, Gwalior, India.
  • Gulab S. Thakur Microbial Biotechnology Laboratory, Tropilite Foods Pvt. Ltd., Davars Campus, Gwalior, India.
  • Pallavi Jaiswal Microbial Biotechnology Laboratory, Tropilite Foods Pvt. Ltd., Davars Campus, Gwalior, India.
  • Anjana Sharma Department of Post Graduate Studies and Research in Biological Sciences, Rani Durgavati Vishwavidyalaya, Jabalpur, India.
  • Prakash S. Bisen Microbial Biotechnology Laboratory, Tropilite Foods Pvt. Ltd., Davars Campus, Gwalior; Department of Post Graduate Studies and Research in Biological Sciences, Rani Durgavati Vishwavidyalaya, Jabalpur, India.

Abstract

The objective of the present study is to develop a lyophilization medium for Streptococcus thermophilus (NCIM 2904) as the industrial exploitation of this bacterium totally depends upon preservation and lyophilization protocols. Protective effect of 18 compounds were observed individually and in combinations with different sugars, sugar alcohols, polymers, protein concentrates and buffers. Among all the protectants tested, ammonium citrate (1% w/w), K2HPO4 (1% w/w) and KH2PO4 (1% w/w) provided lowest protection to these bacterial cells while 10% (w/w) sodium caseinate, whey protein concentrate, sweet whey powder, and skim milk showed significant results in viability escalation. Survival in carbon sources like lactose, sucrose and maltodextrine was also favored maximally. Combination of sodium caseinate 10%, skim milk 5%, sucrose 5%, lactose 5% and mono sodium glutamate 1% in distilled water in ratio of 1:5 with S. thermophilus showed survival percentage of 96%.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.
Published
2014-09-02
Info
Issue
Section
Articles
Keywords:
lyophilization, freeze drying, S. thermophilus, lyoprotectant, viability
Statistics
  • Abstract views: 3702

  • PDF: 1553
  • HTML: 1333
How to Cite
Sharma, R., Sanodiya, B. S., Thakur, G. S., Jaiswal, P., Sharma, A., & Bisen, P. S. (2014). Standardization of lyophilization medium for Streptococcus thermophilus subjected to viability escalation on freeze drying. Microbiology Research, 5(1). https://doi.org/10.4081/mr.2014.5402