Optimization and Immobilization of alpha-amylase from Bacillus subtilis in calcium alginate and calcium alginate – cellulosic residue beads

  • Abdallah Herizi | herizia591@gmail.com Department of Chemistry, Ecole Normale Supérieure de Kouba; Faculty of Technology, Mohamed Boudiaf M’sila University, Algeria.
  • Souilah Rachid Department of Chemistry, Ecole Normale Supérieure de Kouba; Department of Physics, Ecole Normale Supérieure de Laghouat, Algeria.
  • Djabali Djaffar Department of Chemistry, Ecole Normale Supérieure de Kouba, Algeria.
  • Nadjemi Boubekeur Department of Chemistry, Ecole Normale Supérieure de Kouba, Algeria.

Abstract

In this study, Alpha amylase from Bacillus subtilis was immobilized by entrapment in Calcium Alginate beads (CA). To improve the properties of these beads, alginate was blended with Cellulosic Residue (CR) obtained from sorghumstarch extraction. The conditions of entrapment were optimized for a maximum immobilization yield (Y%) by mathematical statistics, where the 23-full factorial design of experiments was used. The properties of calcium alginate beads were improved by comparing the activity of immobilized enzymes in the hydrolysis of starch. The activity of the immobilized enzyme by Calcium Alginate /Cellulosic Residue (CA/CR) was found to be higher than the Calcium Alginate method. Zn2+ and Cu2+ have inhibitory effects on both immobilized enzymes. The Bacillus subtilis immobilized in alginate can be reused for 7 cycles with 12.7 μmol of reduced sugars and 6 cycles for the entrapped enzyme in CA/CR with 30 μmol of reduced sugars.

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Published
2020-03-20
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Research Papers
Keywords:
Alginate, cellulosic residue, α-amylase, enzyme immobilization, optimization
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How to Cite
Herizi, A., Rachid, S., Djaffar, D., & Boubekeur, N. (2020). Optimization and Immobilization of alpha-amylase from <i>Bacillus subtilis</i&gt; in calcium alginate and calcium alginate – cellulosic residue beads. Microbiology Research, 11(1). https://doi.org/10.4081/mr.2020.8458