Lasiodiplodia theobromae keratitis: a rare fungi from eastern India

  • Suman Saha | sumansaha13@gmail.com Ocular Microbiology Services, Priyamvada Birla Aravind Eye Hospital, Kolkata, India.
  • Jayangshu Sengupta Cornea Services, Priyamvada Birla Aravind Eye Hospital, Kolkata, India.
  • Debdulal Banerjee Department of Botany and Forestry, Vidyasagar University, Midnapore, West Bengal, India.
  • Archana Khetan Cornea Services, Priyamvada Birla Aravind Eye Hospital, Kolkata, India.

Abstract

We report here a case of a 32-year old female with a history of minor trauma presented with total corneal ulcer and hypopyon in the left eye. Microbiological examination of corneal scraping showed refractile hyphae with asepted branching filaments and black pigmented colonies in multiple solid agar medium. Identification of the organism was made from culture using D1/D2 region of Large Sub Unit (28S rDNA) based molecular technique. Polymerase chain reaction amplified a band with a sequence that was 100% homologous with Lasiodiplodia theobromae. The organism was sensitive to amphotericin B and voriconazole, and demonstrated resistance to itraconazole and fluconazole. Therapeutic keratoplasty was performed followed by recurrence in graft controlled with topical voriconazole and intracameral amphotericin B. Graft failure was reported after three months.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.

Author Biography

Suman Saha, Ocular Microbiology Services, Priyamvada Birla Aravind Eye Hospital, Kolkata

 

 

Published
2013-01-30
Info
Issue
Section
Brief Reports
Keywords:
keratitis, Lasiodiplodia sp.
Statistics
  • Abstract views: 2889

  • PDF: 376
  • HTML: 14
How to Cite
Saha, S., Sengupta, J., Banerjee, D., & Khetan, A. (2013). Lasiodiplodia theobromae keratitis: a rare fungi from eastern India. Microbiology Research, 3(2), e19. https://doi.org/10.4081/mr.2012.e19