Green synthesis of Silver nanoparticles through Calotropis gigantea leaf extracts and evaluation of antibacterial activity against Vibrio alginolyticus

  • Vaseeharan Baskaralingam | vaseeharanb@gmail.com Dept. Animal Health and Management, Alagappa University, Alagappa Nagar, Tamil Nadu, India.
  • Clara Gunapoorani Sargunar PG & Research Dept. Zoology, Government Arts College (Autonomous), Tamil Nadu, India.
  • Yong Chin Lin Dept. Aquaculture, College of Life Sciences, National Taiwan Ocean University, Taiwan, Taiwan, Province of China.
  • Jiann Chu Chen Dept. Aquaculture, College of Life Sciences, National Taiwan Ocean University, Taiwan, Taiwan, Province of China.

Abstract

Green synthesized silver nanoparticles by Calotropis gigantea leaf extract were used to study the inhibitory activity against pathogenic Vibrio alginolyticus, isolated from wild Artemia franciscana cysts. Silver nanoparticle synthesis was observed using UV-visible spectroscopy and the morphological characteristics were analyzed by atomic force microscope (AFM). In the present study, increasing concentrations of silver nanoparticles synthesized on LB agar plates effectively reduced the number of colonies of V. alginolyticus. A decrease in colonies (CFUs) was observed at 5 mg/mL of silver nanoparticle concentration and the complete inhibition of V. alginolyticus was observed at 20 mg/mL of silver nanoparticle concentration on LB agar plates. In vivo controlling efficiency of silver nanoparticles was tested in an A. franciscana hatching system. Effective control of V. alginolyticus in brine shrimp A. franciscana hatching units was achieved by experimental infection and treatment with silver nanoparticles. Experimental infection studies showed that V. alginolyticus infected Artemia nauplii treated with silver nanoparticles (10 mg/mL) had greater survival (>40%) than silver nanoparticles not treated with nauplii. Based on the findings of this study, it is recommended that low concentrations of green synthesized silver nanoparticles should be further investigated for other potential experimental models to control potential medical pathogens.

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Published
2012-01-16
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Original Articles
Keywords:
silver nanoparticle, Vibrio alginolyticus, aquaculture, Artemia franciscana, Calotropis gigantea.
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How to Cite
Baskaralingam, V., Sargunar, C. G., Lin, Y. C., & Chen, J. C. (2012). Green synthesis of Silver nanoparticles through Calotropis gigantea leaf extracts and evaluation of antibacterial activity against Vibrio alginolyticus. Nanotechnology Development, 2(1), e3. https://doi.org/10.4081/nd.2012.e3