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This experiment was conducted to evaluate the feeding values of ginger, red and black pepper powder with protexin on performance of Japanese quails. A total 240 ten-days-old male Japanese quails with an average weight of 19±5 g were divided into 8 treatments with 3 replicates as randomized factorial design. The quails were fed by basal diet as control diet, 2% ginger, 2% red pepper 2% black pepper powder with or without protexin probiotic respectively. At the end of trial for carcass evaluation 2 birds form each group were slaughtered. Also, some parameters such as feed intake, body weight gains, and feed conversion ratio were calculated and compared together. Some blood parameters such as cholesterol, triglyceride, high density lipoprotein, low density lipoprotein of quail’s blood was determined. In conclusion it seems that inclusion of ginger, red and black pepper powder in quails’ diet at level of 2% may be useful and have significant effects on performance and blood biochemical on Japanese quails.