Chitosan biopolymer: Alternative adhesion factor and scaffold matrix for 2D and 3D neuronal cultures


https://doi.org/10.4081/bse.107
Vol. 1 No. 1 (2020)
Published: 14 February 2020
Chitosan, adhesion factor, scaffold, neuronal network
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Authors

  • Donatella Di Lisa Department of Informatics, Bioengineering, Robotics and System Engineering, University of Genoa, Genoa, Italy.
  • Mariateresa Tedesco Department of Informatics, Bioengineering, Robotics and System Engineering, University of Genoa, Genoa, Italy.
  • Elena Dellacasa Department of Informatics, Bioengineering, Robotics and System Engineering, University of Genoa, Genoa, Italy.
  • Mattia Pesce Nanoscopy and Nikon Centre, Istituto Italiano di Tecnologia, Genoa, Italy.
  • Tiziano Catelani Electron Microscopy Facility, Istituto Italiano di Tecnologia, Genoa, Italy.
  • Paolo Massobrio Department of Informatics, Bioengineering, Robotics and System Engineering, University of Genoa, Genoa, Italy.
  • Roberto Raiteri Department of Informatics, Bioengineering, Robotics and System Engineering, University of Genoa, Genoa, Italy.
  • Sergio Martinoia Department of Informatics, Bioengineering, Robotics and System Engineering, University of Genoa, Genoa, Italy.
  • Laura Pastorino
    Laura.pastorino@unige.it
    Department of Informatics, Bioengineering, Robotics and System Engineering, University of Genoa, Genoa, Italy.

The increase of different types of cell cultures, which can be used for the in vitro studies of physiological and/or pathological processes, has introduced the need to improve culture techniques through the use of materials and culture media that promote growth, recreating a cellular micro-environment that can be asserted in in vivo condition. The standard methods for the functionalization of supports used for cell cultures are based on the use of synthetic or natural biopolymers, which generally have high costs, such as poly-lysine and polyornithine. The aim of this work is to demonstrate the alternative use of the polysaccharide chitosan as adhesion factor and structural component for 2D/3D neuronal cultures. Thanks to its versatility, it could be easily functionalized for the fabrication of personalized of in vitro models


Di Lisa, D., Tedesco, M., Dellacasa, E., Pesce, M., Catelani, T., Massobrio, P., Raiteri, R., Martinoia, S., & Pastorino, L. (2020). Chitosan biopolymer: Alternative adhesion factor and scaffold matrix for 2D and 3D neuronal cultures. Biomedical Science and Engineering, 1(1). https://doi.org/10.4081/bse.107

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