A modified Phenol-chloroform extraction method for isolating circulating cell free DNA of tumor patients

  • Clemens Hufnagl | cl.hufnagl@salk.at Third Medical Department with Hematology, Medical Oncology, Hemostaseology, Rheumatology and Infectious Diseases Oncologic Center, Laboratory of Immunological and Molecular Cancer Research (LIMCR), Center for Clinical Cancer and Immunology Trials, Private Medical University Hospital Salzburg, Austria.
  • Markus Stöcher Third Medical Department with Hematology, Medical Oncology, Hemostaseology, Rheumatology and Infectious Diseases Oncologic Center, Laboratory of Immunological and Molecular Cancer Research (LIMCR), Center for Clinical Cancer and Immunology Trials, Private Medical University Hospital Salzburg, Austria.
  • Martin Moik Third Medical Department with Hematology, Medical Oncology, Hemostaseology, Rheumatology and Infectious Diseases Oncologic Center, Laboratory of Immunological and Molecular Cancer Research (LIMCR), Center for Clinical Cancer and Immunology Trials, Private Medical University Hospital Salzburg, Austria.
  • Roland Geisberger Third Medical Department with Hematology, Medical Oncology, Hemostaseology, Rheumatology and Infectious Diseases Oncologic Center, Laboratory of Immunological and Molecular Cancer Research (LIMCR), Center for Clinical Cancer and Immunology Trials, Private Medical University Hospital Salzburg, Austria.
  • Richard Greil Third Medical Department with Hematology, Medical Oncology, Hemostaseology, Rheumatology and Infectious Diseases Oncologic Center, Laboratory of Immunological and Molecular Cancer Research (LIMCR), Center for Clinical Cancer and Immunology Trials, Private Medical University Hospital Salzburg, Austria.

Abstract

Searching for new cancer biomarkers, circulating cell-free DNA (cfDNA) has become an appealing target of interest as an elevated level of cfDNA has been detected in the circulation of cancer patients in comparison with healthy controls. Since cfDNA can be isolated from the circulation and other body fluids of patients without harming their physical condition, cfDNA is becoming a promising candidate as a novel non-invasive biomarker for cancer. The challenge in the diagnostic analysis of cfDNA is its very low presence in human plasma/serum and its partially strong fragmentation. Here we evaluated a modified phenol/chloroform extraction method for the isolation of cfDNA and compared it with published standard methods for cfDNA isolation.

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Published
2013-03-11
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Section
Protocols
Keywords:
cell-free DNA
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How to Cite
Hufnagl, C., StöcherM., Moik, M., Geisberger, R., & Greil, R. (2013). A modified Phenol-chloroform extraction method for isolating circulating cell free DNA of tumor patients. Journal of Nucleic Acids Investigation, 4(1), e1. https://doi.org/10.4081/jnai.2013.4282