In vitro cloning of Bambusa pallida Munro through axillary shoot proliferation and evaluation of genetic fidelity by random amplified polymorphic DNA markers

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Beena D.B. *
Rathore T.S.
(*) Corresponding Author:
Beena D.B. | beena2631@yahoo.com

Abstract

Multiple shoots emerged from the nodal shoot segments of the field-grown candidate plus clump explants of Bambusa pallida Munro when cultured on Murashige and Skoog (MS) liquid medium with additives (ascorbic acid 50 mg/L + citric acid 25 mg/L + cysteine 25 mg/L) and combined use of α-naphthalene acetic acid (NAA) 1.34 μM + thiodiozuron 1.125 μM in a 2-week period. Further shoot multiplication was achieved in MS liquid medium with additives + NAA 1.34 μM + 6-benzylaminopurine 4.4 μM at 25±2°C and 33.78 μmol photons m-2 s-1 light illumination for a 12-h photoperiod. These shoots were rooted within four weeks in MS/2 basal salt medium with additives + 2% sucrose +1% glucose, and 0.6% agar by pulse treatment of shoots with indole 3 butyric acid 0.5 mg/mL for 30 min prior to inoculation. Rooted plants were successfully hardened in the mist chamber. Survival rate during hardening was more than 95%. Micropropagated plants achieved a height of 25-30 cm with 3-4 tillers (shoots) with miniature rhizome in a 4-month period. Genetic stability was observed in the micropropagated plants.

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