An automated continuous monitoring system: a useful tool for monitoring neuronal differentiation of human embryonic stem cells

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Tuomas Tapani Huttunen
Maria Sundberg
Harri Pihlajamäki
Riitta Suuronen
Heli Skottman
Riikka Äänismaa
Susanna Narkilahti *
(*) Corresponding Author:
Susanna Narkilahti | susanna.narkilahti@uta.fi

Abstract

The currently used cell culturing and differentiation procedures are both time- and laborintensive. Automation of some of these procedures will increase the efficiency of commonly used cell differentiation protocols. We used a particular cell culture platform to rapidly and efficiently screen the neuronal differentiation of human embryonic stem cells (hESC). Continuous live monitoring and analysis of non-labeled cells using this system allowed us to characterize neuronal populations over the entire neuronal differentiation process. The differentiation of individual cells from early progenitor cells to neurons and glial cells could be monitored continuously using this system with sub-confluent cell cultures. The imaged data was collected and analyzed with a specially designed cell recognition protocol, which resulted in a quantitative neuronal cell count. The analysis results were confirmed using conventional laboratory methods such as manual counting and flow cytometry. Our findings suggest that an automated culture platform combined with automated monitoring and analysis systems is a reliable method for developing enhanced cell differentiation procedures or as part of an automated quality control system for existing protocols.

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