Reconstituted epithelial corneal tissues for evaluation of drug delivery

  • Susi Burgalassi | susi.burgalassi@unipi.it Department of Pharmacy, University of Pisa, Pisa; Italian inter-university centre for the promotion of the 3Rs in teaching and research, Pisa, Italy.
  • Erica Zucchetti Department of Pharmacy, University of Pisa, Pisa; Italian inter-university centre for the promotion of the 3Rs in teaching and research, Pisa, Italy.
  • Eleonora Terreni Department of Pharmacy, University of Pisa, Pisa; Italian inter-university centre for the promotion of the 3Rs in teaching and research, Pisa, Italy.
  • Daniela Monti Department of Pharmacy, University of Pisa, Pisa; Italian inter-university centre for the promotion of the 3Rs in teaching and research, Pisa, Italy.
  • Silvia Tampucci Department of Pharmacy, University of Pisa, Pisa; Italian inter-university centre for the promotion of the 3Rs in teaching and research, Pisa, Italy.
  • Patrizia Chetoni Department of Pharmacy, University of Pisa, Pisa; Italian inter-university centre for the promotion of the 3Rs in teaching and research, Pisa, Italy.

Abstract

One possible approach to reduce the use of animals in the evaluation of the ocular drug delivery is that to use cell cultures as model of tissues. In this study are compared two different reconstituted epithelial corneal tissues, a homemade artificial corneal epithelium (Reconstituted Rabbit Corneal Epithelium) and a commercial human corneal epithelial model (COR-100 EpiCornealTM, MatTek), for permeation experiments of three drugs with different physical chemical properties: timolol maleate, cyclosporin A, and a newly synthesized compound, MAGL 17.b. The collected data show that corneal epithelial models are not sufficient to simulate the complexity of the corneal barrier and the presence of a layer simulating the stroma may be necessary to approach the structure of native cornea.

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Published
2020-02-11
Keywords:
Corneal permeation, reconstituted tissues, drug partition coefficient
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How to Cite
Burgalassi, S., Zucchetti, E., Terreni, E., Monti, D., Tampucci, S., & Chetoni, P. (2020). Reconstituted epithelial corneal tissues for evaluation of drug delivery. Biomedical Science and Engineering, 3(s2). https://doi.org/10.4081/bse.2019.82